Professor

Bridgeside Point, 100 Technology Drive
Room 332, BRIDG
Pittsburgh, PA 15219-3130
Phone: (412) 624-8864
Email: aab20@pitt.edu

Personal Web Page

Research Interests
Publications
Education
My Links

Research Interests

The primary focus of current research is investigating the cellular and molecular mechanisms underlying human blood vessel and lung diseases caused by environmental exposures to metals and chronic changes in redox status. In vivo and cell cultured based studies focus on the molecular pathology and etiology of vascular disease caused by chronic exposure to low levels of arsenic in drinking water. The cell signaling pathways that mediate arsenic stimulated pathogenic phenotypic changes in endothelial cells are being investigated. Additional studies examine the molecular signaling mechanisms mediating gene induction and silencing in airway epithelial cells exposed to chromium. The objective of these studies is to identify the pathways through which inhaled chromium aggravates lung injury from infections and exposure to other metals.

Publications

• Barchowsky A, Buckley LA, Carlson GP, Fitsanakis VA, Ford SM, Genter MB, Germolec DR, Leavens TL, Lehman-McKeeman LD, Safe SH, Sulentic CE, Eidemiller BJ.
  The Toxicology Education Summit: building the future of toxicology through education.
  Toxicol Sci. 2012 Mar 29. PMID: 22461448
• Gao, F. Brant, K.A., Ward, R.M., Cattley, R.T., Barchowsky, A., Brant, K., and Fabisiak, J.P.
  Multiple protein kinase pathways mediate amplified IL-6 release by human lung fibroblasts co-exposed to nickel and TLR-2 agoinst, MALP-2.
  Toxicol. Appl. Pharmacol. 2010 Sep 1;247(2):146-57. Epub 2010 Jun 17. PubMed PMID: 20600219
• Liu FJ, Barchowsky A, Opresko PL.
  The Werner Syndrome Protein Suppresses Telomeric Instability Caused by Chromium (VI) Induced DNA Replication Stress.
  PLoS One. 2010 Jun 16;5(6):e11152. PMID: 20585393
• Jang AS, Concel VJ, Bein K, Brant KA, Liu S, Pope-Varsalona H, Dopico Jr. RA, Di YP, Knoell DL, Barchowsky A, Leikauf GD.
  Endothelial Dysfunction and Claudin 5 Regulation during Acrolein-induced Lung Injury.
  Am J Respir Cell Mol Biol. 2011 Apr;44(4):483-90. Epub 2010 Jun 4. PubMed PMID: 20525806
• Nemec AA, Zubritsky LM, Barchowsky A.
  Chromium(VI) Stimulates Fyn to Initiate Innate Immune Gene Induction in Human Airway Epithelial Cells.
  Chem Res Toxicol. 2009 Dec 8. PMID: 19994902
• Liu FJ, Barchowsky A, Opresko PL.
  The Werner Syndrome Protein Functions in Repair of Cr (VI)-induced Replication.
  Associated DNA Damage. Toxicol Sci. 2009 Jun 1. PMID: 19487340
• Nemec AA, Barchowsky A.
  Signal transducer and activator of transcription 1 (STAT1) is essential for chromium silencing of gene induction in human airway epithelial cells.
  Toxicol Sci. 2009 Apr 29. PMID: 19403854
• Straub AC, LR Klei, DB Stolz, A Barchowsky.
  Arsenic requires sphingosine-1-phosphate type 1 receptors to induce angiogenic genes and endothelial cell remodeling.
  Am J Pathol 174, May 2009. PMID: 19349368
• Bein K, Wesselkamper SC, Liu X, Dietsch M, Majumder N, Concel VJ, Medvedovic M, Sartor M, Henning LN, Venditto C, Borchers MT, Barchowsky A, Weaver TE, Tichelaar JW, Prows DR, Korfhagen TR, Hardie WD, Bachurski CJ, Leikauf GD.
  Surfactant Associated Protein B is Critical to Survival in Nickel-induced Injury in Mice.
  Am J Respir Cell Mol Biol. 2009 Jan 8. PMID: 19131640
• Nemec AA, Leikauf G, Pitt BR, Wasserloos KJ, Barchowsky A.
  Nickel Mobilizes Intracellular Zinc to Induce Metallothionein in Human Airway Epithelial Cells.
  Am J Respir Cell Mol Biol. 2008 Dec 18. PMID: 19097988
• States JC, Srivastava S, Chen Y, Barchowsky A.
  Arsenic and Cardiovascular Disease.
  Toxicol Sci. 2008 Nov 17. PMID: 19015167
• Straub AC, Clark KA, Ross MA, Chandra AG, Li S, Gao X, Pagano PJ, Stolz DB, Barchowsky A.
  Arsenic-stimulated liver sinusoidal capillarization in mice requires NADPH oxidase-generated superoxide.
  J Clin Invest. 2008 Nov 13. PMID: 19009047
• Dougherty D, Garte S, Barchowsky A, Zmuda J, Taioli E
  NQO1, MPO, CYP2E1, GSTT1 and GSTM1 polymorphisms and biological effects of benzene exposure-A literature review.
  Toxicol Lett. 2008 Sep 20. PMID: 18848868.
• Basu P, Ghosh RN, Grove LE, Klei L, Barchowsky A.
  Angiogenic potential of 3-nitro-4-hydroxy benzene arsonic Acid (roxarsone).
  Environ Health Perspect. 2008 Apr;116(4):520-3. PMID: 18414637
• Klei, L R and Barchowsky, A.
  Positive signaling interactions between arsenic and ethanol for angiogenic gene induction in human microvascular endothelial cells.
  Toxicological Science Advance Access published on January 8, 2008. doi:10.1093/toxsci/kfn003 PMID: 18182400
• Prozialeck WC, Edwards JR, Nebert DW, Woods JM, Barchowsky A, Atchison WD.
  The Vascular System as a Target of Metal Toxicity.
  Toxicol Sci. 2007 Oct 18. PMID: 17947343
• Shvedova, A.A., Fabisiak, J.P., Kisin, E.R., Murray, A., Roberts, J., Antonini, J., Kommineini, C., Reynolds, J., Barchwosky, A., Castranova, V., and Kagan, V.E.
  Sequential exposure to carbon nanotubes and bacteria enhances pulmonary inflammation and infectivity.
  Am. J. Respir. Cell Molec. Biol., 38: 579-90, 2008. PMID: 18096873
• Zhao, J, RW Harper, A Barchowsky, YP Di.
  Identification of multiple MAPK-mediated transcription factors regulated by tobacco smoke in airway epithelial cells.
  Am J Physiol Lung Cell Mol Physiol 293:L480-l490, 2007. PMID: 17496060
• Shenberger JS, L Zhang, RJ Powell, and A Barchowsky.
  Hyperoxia enhances VEGF release from A549 cells via post-transcriptional processes.
  Free Radic Biol Med. 43:844-852, 2007. PMID: 17664148
• Straub, AC, DB Stolz, H. Vin, MA Ross, NV Soucy, LR Klei, and A Barchowsky.
  Low level arsenic promotes progressive inflammatory angiogenesis and liver blood vessel remodeling in mice.
  Toxicol. Appl. Pharmacol. 222:327-336, 2007 PMID: 17123562
• Straub, AC, DB Stolz, MA Ross, NV Soucy, LR Klei and A Barchowsky.
  Arsenic stimulates sinusoidal endothelial cell capillarization and vessel remodeling in mouse liver.
  Hepatology 45:205-212, 2007 PMID: 17187425
• O'Hara, KA, R J Vaghjiani, AA Nemec, LR Klei and A Barchowsky.
  Chromium(VI)-stimulated STAT3 tyrosine phosphorylation and nuclear translocation in human airway epithelial cells requires Lck.
  Biochem J. 402:261-269, 2007 PMID: 17078813

Education

• BS Zoology, North Carolina State University, Raleigh, NC 1978
• PhD Pharmacology, Duke University, Durham, NC 1984

My Links

GSPH Environmental and Occupational Health